cPLA2 (phospho Ser505) Polyclonal Antibody

    • Catalog No.:YP0868
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • PLA2G4A
      • Protein Name:
      • Cytosolic phospholipase A2
      • Human Gene Id:
      • 5321
      • Human Swiss Prot No:
      • P47712
      • Mouse Swiss Prot No:
      • P47713
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520
      • Specificity:
      • Phospho-cPLA2 (S505) Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at S505.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA
      • MolecularWeight(Da):
      • 85211
      • Observed Band(KD):
      • 110
      • Background:
      • phospholipase A2 group IVA(PLA2G4A) Homo sapiens This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],
      • Function:
      • catalytic activity:2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.,catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,domain:The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca(2+).,function:Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.,PTM:Activated by phosphorylation at both Ser-505 and Ser-727.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane ve
      • Subcellular Location:
      • cytoplasm,mitochondrial inner membrane,endoplasmic reticulum membrane,Golgi apparatus,cytosol,cytoplasmic, membrane-bounded vesicle,cytoplasmic vesicle,
      • Expression:
      • Epithelium,Platelet,
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,cPLA2 (phospho Ser505) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
      • Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-PLA2 (Phospho-Ser505) Antibody
      • Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 30', using c-PLA2 (Phospho-Ser505) Antibody. The lane on the right is blocked with the phospho peptide.