14-3-3 ζ (phospho Ser58) Polyclonal Antibody

    • Catalog No.:YP0853
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • YWHAZ
      • Protein Name:
      • 14-3-3 protein zeta/delta
      • Human Gene Id:
      • 7534
      • Human Swiss Prot No:
      • P63104
      • Mouse Swiss Prot No:
      • P63101
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73
      • Specificity:
      • Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
      • MolecularWeight(Da):
      • 27745
      • Observed Band(KD):
      • 30
      • Background:
      • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta(YWHAZ) Homo sapiens This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
      • Function:
      • caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to
      • Subcellular Location:
      • extracellular space,nucleus,nucleoplasm,cytoplasm,mitochondrion,cytosol,cell-cell adherens junction,focal adhesion,cytoplasmic vesicle membrane,melanosome,extracellular exosome,
      • Expression:
      • B-cell lymphoma,Bone marrow
      • Products Images
      • Western Blot analysis of various cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 zeta (Phospho-Ser58) Antibody
      • Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.