RARα (phospho Ser77) Polyclonal Antibody

    • Catalog No.:YP0803
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • RARA
    • Protein Name:
    • Retinoic acid receptor alpha
    • Human Gene Id:
    • 5914
    • Human Swiss Prot No:
    • P10276
    • Mouse Swiss Prot No:
    • P11416
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of Ser77. AA range:46-95
    • Specificity:
    • Phospho-RARα (S77) Polyclonal Antibody detects endogenous levels of RARα protein only when phosphorylated at S77.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • RARA; NR1B1; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1
    • MolecularWeight(Da):
    • 50771
    • Observed Band(KD):
    • 45
    • Background:
    • RARA (retinoic acid receptor alpha)represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. RARA has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
    • Function:
    • regulation of cytokine production, negative regulation of cytokine production, positive regulation of cytokine production, positive regulation of immune system process, regulation of leukocyte activation, positive regulation of leukocyte activation, regulation of adaptive immune response, regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains, regulation of T-helper 2 type immune response, transcription, regulation of transcription, DNA-dependent, intracellular signaling cascade,response to nutrient, response to endogenous stimulus, response to hormone stimulus, response to extracellular stimulus, response to organic substance, steroid hormone receptor signaling pathway, estrogen receptor signaling pathway, intracellular receptor-mediated signaling pathway, response to nutrient levels, response to estradiol
    • Products Images
    • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The picture on the right is blocked with the phospho peptide.
    • Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.