• Target：
• GRK1

• Fields：
• >>Chemokine signaling pathway;>>Endocytosis;>>Phototransduction

• Gene Name：
• GRK1

• Protein Name：
• Rhodopsin kinase

• Human Gene Id：
• 6011

• Human Swiss Prot No：
• Q15835

• Mouse Swiss Prot No：
• Q9WVL4

• Rat Gene Id：
• 81760

• Rat Swiss Prot No：
• Q63651

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human GRK1 around the phosphorylation site of Ser21. AA range:6-55

• Specificity：
• Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• GRK1;RHOK;Rhodopsin kinase;RK;G protein-coupled receptor kinase 1

• Observed Band(KD)：
• 63kD

• Background：
• This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.,disease:Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type (CSNBO) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation.,function:Phosphorylates rhodopsin thereby initiating its deactivation.,online information:Retina International's Scientific Newsletter,PTM:Autophosphorylated.,PTM:Farnesylation is required for full activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,tissue specificity:R

• Subcellular Location：
• Membrane ; Lipid-anchor . Cell projection, cilium, photoreceptor outer segment . Subcellular location is not affected by light or dark conditions. .

• Expression：
• Retinal-specific. Expressed in rods and cones cells.

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