• Target：
• p130 Cas

• Fields：
• >>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection

• Gene Name：
• BCAR1

• Protein Name：
• Breast cancer anti-estrogen resistance protein 1

• Human Gene Id：
• 9564

• Human Swiss Prot No：
• P56945

• Mouse Gene Id：
• 12927

• Mouse Swiss Prot No：
• Q61140

• Rat Gene Id：
• 25414

• Rat Swiss Prot No：
• Q63767

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr165. AA range:131-180

• Specificity：
• Phospho-p130 Cas (Y165) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y165.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BCAR1;CAS;CASS1;CRKAS;Breast cancer anti-estrogen resistance protein 1;CRK-associated substrate;Cas scaffolding protein family member 1;p130cas

• Observed Band(KD)：
• 130kD

• Background：
• BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],

• Function：
• domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphoryl

• Subcellular Location：
• Cell junction, focal adhesion . Cytoplasm . Cell projection, axon . Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity). .

• Expression：
• Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

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