eEF2K (phospho Ser366) Polyclonal Antibody

    • Catalog No.:YP0599
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Rat
      • Target:
      • eEF2K
      • Fields:
      • >>AMPK signaling pathway;>>Oxytocin signaling pathway
      • Gene Name:
      • EEF2K
      • Protein Name:
      • Eukaryotic elongation factor 2 kinase
      • Human Swiss Prot No:
      • O00418
      • Mouse Swiss Prot No:
      • O08796
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380
      • Specificity:
      • Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • EEF2K;Eukaryotic elongation factor 2 kinase;eEF-2 kinase;eEF-2K;Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
      • Observed Band(KD):
      • 105kD
      • Background:
      • This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer .,
      • Subcellular Location:
      • cytoplasm,cytosol,postsynaptic density,
      • Expression:
      • Epithelium,Glial tumor,Lymph,T-cell,
      • Products Images
      • Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2K (Phospho-Ser366) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HeLa cells treated with serum 10% 15', using eEF2K (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.