• Target：
• eEF2K

• Fields：
• >>AMPK signaling pathway;>>Oxytocin signaling pathway

• Gene Name：
• EEF2K

• Protein Name：
• Eukaryotic elongation factor 2 kinase

• Human Gene Id：
• 29904

• Human Swiss Prot No：
• O00418

• Mouse Swiss Prot No：
• O08796

• Rat Gene Id：
• 25435

• Rat Swiss Prot No：
• P70531

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380

• Specificity：
• Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EEF2K;Eukaryotic elongation factor 2 kinase;eEF-2 kinase;eEF-2K;Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

• Observed Band(KD)：
• 105kD

• Background：
• This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer .,

• Subcellular Location：
• cytoplasm,cytosol,postsynaptic density,

• Expression：
• Epithelium,Glial tumor,Lymph,T-cell,

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