CD31 (phospho Tyr713) Polyclonal Antibody

    • Catalog No.:YP0530
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • PECAM1
      • Protein Name:
      • Platelet endothelial cell adhesion molecule
      • Human Gene Id:
      • 5175
      • Human Swiss Prot No:
      • P16284
      • Mouse Swiss Prot No:
      • Q08481
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735
      • Specificity:
      • Phospho-CD31 (Y713) Polyclonal Antibody detects endogenous levels of CD31 protein only when phosphorylated at Y713.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
      • MolecularWeight(Da):
      • 82536
      • Observed Band(KD):
      • 150
      • Background:
      • platelet and endothelial cell adhesion molecule 1(PECAM1) Homo sapiens The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],
      • Function:
      • function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,
      • Subcellular Location:
      • extracellular space,plasma membrane,external side of plasma membrane,integral component of membrane,cell junction,smooth muscle contractile fiber,platelet alpha granule membrane,membrane raft,extracellular exosome,
      • Expression:
      • Brain,Liver,Platelet,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PECAM-1 (Phospho-Tyr713) Antibody
      • Immunofluorescence analysis of HeLa cells, using PECAM-1 (Phospho-Tyr713) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HepG2 cells, using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is blocked with the phospho peptide.