CD31 (phospho Tyr713) Polyclonal Antibody
- Catalog No.:YP0530
- Applications:WB,IHC-p,IF/ICC,ELISA
- Reactivity:Human,Mouse
- Gene Name:
- PECAM1
- Protein Name:
- Platelet endothelial cell adhesion molecule
- Human Gene Id:
- 5175
- Human Swiss Prot No:
- P16284
- Mouse Gene Id:
- 18613
- Mouse Swiss Prot No:
- Q08481
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735
- Specificity:
- Phospho-CD31 (Y713) Polyclonal Antibody detects endogenous levels of CD31 protein only when phosphorylated at Y713.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Rabbit
- Dilution:
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
- MolecularWeight(Da):
- 82536
- Observed Band(KD):
- 150
- Background:
- platelet and endothelial cell adhesion molecule 1(PECAM1) Homo sapiens The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],
- Function:
- function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,
- Subcellular Location:
- extracellular space,plasma membrane,external side of plasma membrane,integral component of membrane,cell junction,smooth muscle contractile fiber,platelet alpha granule membrane,membrane raft,extracellular exosome,
- Expression:
- Brain,Liver,Platelet,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PECAM-1 (Phospho-Tyr713) Antibody
- Immunofluorescence analysis of HeLa cells, using PECAM-1 (Phospho-Tyr713) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HepG2 cells, using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is blocked with the phospho peptide.