• Target：
• EphA4

• Fields：
• >>Axon guidance

• Gene Name：
• EPHA4

• Protein Name：
• Ephrin type-A receptor 4

• Human Gene Id：
• 2043

• Human Swiss Prot No：
• P54764

• Mouse Gene Id：
• 13838

• Mouse Swiss Prot No：
• Q03137

• Immunogen：
• Synthesized phospho-peptide around the phosphorylation site of human EphA4 (phospho Tyr596)

• Specificity：
• Phospho-EphA4 (Y596) Polyclonal Antibody detects endogenous levels of EphA4 protein only when phosphorylated at Y596.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EPHA4;HEK8;SEK;TYRO1;Ephrin type-A receptor 4;EPH-like kinase 8;EK8;hEK8;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK

• Observed Band(KD)：
• 110kD

• Background：
• This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:The protein kinase domain mediates interaction with NGEF/ephexin-1.,function:Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:Interacts with the src family kinase, p59-Fyn, through the major phosphorylation site at position Tyr-602. Interacts with NGEF/ephexin-1.,tissue specificity:Ubiquitous.

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, postsynaptic density membrane . Early endosome . Cell junction, adherens junction . Clustered upon activation and targeted to early endosome. .

• Expression：
• Ubiquitous.

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