• Target：
• 14-3-3 β/ζ

• Fields：
• >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis

• Gene Name：
• YWHAB/YWHAZ

• Protein Name：
• 14-3-3 protein beta/alpha/14-3-3 protein zeta/delta

• Human Gene Id：
• 7534/7529

• Human Swiss Prot No：
• P63104/P31946

• Mouse Gene Id：
• 22631/54401

• Rat Gene Id：
• 25578/56011

• Rat Swiss Prot No：
• P63102/P35213

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of Ser184/186. AA range:151-200

• Specificity：
• Phospho-14-3-3 β/ζ (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein only when phosphorylated at S184/186.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• YWHAZ;14-3-3 protein zeta/delta;Protein kinase C inhibitor protein 1;KCIP-1;YWHAB;14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1

• Observed Band(KD)：
• 28kD

• Background：
• This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

• Function：
• caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to

• Subcellular Location：
• Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.

• Expression：
• B-cell lymphoma,Bone marrow

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