Myf-5 (phospho Ser49) Polyclonal Antibody
- Catalog No.:YP0395
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- Myf-5
- Fields:
- >>Signaling pathways regulating pluripotency of stem cells
- Gene Name:
- MYF5
- Protein Name:
- Myogenic factor 5
- Human Gene Id:
- 4617
- Human Swiss Prot No:
- P13349
- Mouse Gene Id:
- 17877
- Mouse Swiss Prot No:
- P24699
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70
- Specificity:
- Phospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MYF5;BHLHC2;Myogenic factor 5;Myf-5;Class C basic helix-loop-helix protein 2;bHLHc2
- Observed Band(KD):
- 38kD
- Background:
- function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
- Function:
- function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
- Subcellular Location:
- Nucleus .
- Expression:
- Skeletal muscle,
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.