Cdk5 (phospho Tyr15) Polyclonal Antibody

    • Catalog No.:YP0380
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
    • Description
    • Gene Name:
    • CDK5
    • Protein Name:
    • Cyclin-dependent kinase 5
    • Human Gene Id:
    • 1020
    • Human Swiss Prot No:
    • Q00535
    • Mouse Swiss Prot No:
    • P49615
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human CDK5 around the phosphorylation site of Tyr15. AA range:1-50
    • Specificity:
    • Phospho-Cdk5 (Y15) Polyclonal Antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Y15.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • CDK5; CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
    • MolecularWeight(Da):
    • 33304
    • Observed Band(KD):
    • 33
    • Background:
    • CDK5 encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the cyclin dependent kinase 5 encoded by CDK5 does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.
    • Function:
    • cell morphogenesis, cell morphogenesis involved in differentiation, neuron migration, synaptic transmission, dopaminergic, protein complex assembly, protein amino acid phosphorylation, phosphorus metabolic process,phosphate metabolic process, cellular ion homeostasis, intracellular protein transport, exocytosis, endocytosis,apoptosis, cell motion, cytoskeleton organization, cell cycle, cell adhesion, cell-matrix adhesion, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway, signal complex assembly, intracellular signaling cascade, protein kinase cascade,JAK-STAT cascade, cell-cell signaling, synaptic transmission, nerve-nerve synaptic transmission, axonogenesis, axon guidance, peripheral nervous system development, muscle organ development, skeletal muscle tissue development,s
    • Products Images
    • Western Blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody
    • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
    • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK5 (Phospho-Tyr15) Antibody
    • Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u, 15mins, using CDK5 (Phospho-Tyr15) Antibody. The lane on the right is blocked with the phospho peptide.