Cdk5 (phospho Tyr15) Polyclonal Antibody

    • Catalog No.:YP0380
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
    • Description
    • Gene Name:
    • CDK5
    • Protein Name:
    • Cyclin-dependent kinase 5
    • Human Gene Id:
    • 1020
    • Human Swiss Prot No:
    • Q00535
    • Mouse Swiss Prot No:
    • P49615
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human CDK5 around the phosphorylation site of Tyr15. AA range:1-50
    • Specificity:
    • Phospho-Cdk5 (Y15) Polyclonal Antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Y15.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • CDK5; CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
    • MolecularWeight(Da):
    • 33304
    • Observed Band(KD):
    • 33
    • Background:
    • cyclin dependent kinase 5(CDK5) Homo sapiens This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015],
    • Function:
    • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:In axonal growth cone with extension to the peripheral lamellipodia.,subunit:Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK.,
    • Subcellular Location:
    • nucleus,nucleoplasm,cytoplasm,cytosol,cytoskeleton,plasma membrane,postsynaptic density,membrane,cyclin-dependent protein kinase 5 holoenzyme complex,lamellipodium,cell junction,filopodium,axon,dendrite,
    • Expression:
    • Fetal brain,Lung,
    • Products Images
    • Western Blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody
    • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
    • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK5 (Phospho-Tyr15) Antibody
    • Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u, 15mins, using CDK5 (Phospho-Tyr15) Antibody. The lane on the right is blocked with the phospho peptide.