Cdk5 (phospho Tyr15) Polyclonal Antibody

    • Catalog No.:YP0380
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
    • Description
    • Gene Name:
    • CDK5
    • Protein Name:
    • Cyclin-dependent kinase 5
    • Human Gene Id:
    • 1020
    • Human Swiss Prot No:
    • Q00535
    • Mouse Swiss Prot No:
    • P49615
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human CDK5 around the phosphorylation site of Tyr15. AA range:1-50
    • Specificity:
    • Phospho-Cdk5 (Y15) Polyclonal Antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Y15.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • CDK5; CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
    • MolecularWeight(Da):
    • 33304
    • Observed Band(KD):
    • 33
    • Background:
    • CDK5 encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the cyclin dependent kinase 5 encoded by
    • Function:
    • cell morphogenesis, cell morphogenesis involved in differentiation, neuron migration, synaptic trans
    • Products Images
    • Western Blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody
    • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
    • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK5 (Phospho-Tyr15) Antibody
    • Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u, 15mins, using CDK5 (Phospho-Tyr15) Antibody. The lane on the right is blocked with the phospho peptide.