• Target：
• JAK2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Cholinergic synapse;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Leishmaniasis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis

• Gene Name：
• JAK2

• Protein Name：
• Tyrosine-protein kinase JAK2

• Human Gene Id：
• 3717

• Human Swiss Prot No：
• O60674

• Mouse Gene Id：
• 16452

• Mouse Swiss Prot No：
• Q62120

• Rat Gene Id：
• 24514

• Rat Swiss Prot No：
• Q62689

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr570. AA range:541-590

• Specificity：
• Phospho-JAK2 (Y570) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y570.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• JAK2;Tyrosine-protein kinase JAK2;Janus kinase 2;JAK-2

• Observed Band(KD)：
• 130kD

• Background：
• This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.,disease:Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.,disease:Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients incl

• Subcellular Location：
• Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .

• Expression：
• Ubiquitously expressed throughout most tissues.

Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. Nature Communications Nat Commun. 2020 Apr;11(1):1-16 WB Mouse 1:1000 BMDMs

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3. Journal of Neuroinflammation J Neuroinflamm. 2021 Dec;18(1):1-20 IHC,WB Rat 1:100 ,1:1000 Red nucleus (RN)

Mitochondrial dysfunction-mediated decline in angiogenic capacity of endothelial progenitor cells is associated with capillary rarefaction in patients with hypertension via downregulation of CXCR4/JAK2/SIRT5 signaling. EBioMedicine Ebiomedicine. 2019 Apr;42:64 WB Rat 1:1000 EPCs

Effects and Molecular Mechanism of Single-Nucleotide Polymorphisms of MEG3 on Porcine Skeletal Muscle Development. Frontiers in Genetics Front Genet. 2021 Feb;12:206 WB Porcine 1 : 1000 Porcine skeletal muscle satellite cells

miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3 Journal of Oncology Bing Wu WB Mouse

Progranulin promoted the proliferation, metastasis, and suppressed apoptosis via JAK2-STAT3/4 signaling pathway in papillary thyroid carcinoma. Cancer Cell International Zhen Liu WB Human 1:800 BCPAP cell,TPC1 cell

Shenlian extract improves atherosclerosis by relieving adventitial inflammation. JOURNAL OF ETHNOPHARMACOLOGY Xiaoxin Zhu WB Rat vascular adventitial fibroblasts (VAFs)

Overexpression of miR-506-3p reversed doxorubicin resistance in drug-resistant osteosarcoma cells Frontiers in Pharmacology Wang Xinru WB Human 1:1000 U-2OS/Dox cell

PCV2 Induced Endothelial Derived IL-8 Affects MoDCs Maturation Mainly via NF-κB Signaling Pathway Viruses-Basel Mengyu Zhang WB Pig monocyte-derived dendritic cell (MoDC),porcine hip artery endothelial cell (PIEC)

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