mTOR (phospho Ser2448) Polyclonal Antibody

  • Catalog No.：YP0176
  • Applications：WB;IHC;IF;ELISA
  • Reactivity：Human;Mouse;Rat;Bovine;Pig
    • Target：
    • mTOR
    • Fields：
    • >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - other;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Apelin signaling pathway;>>Neutrophil extracellular trap formation;>>JAK-STAT signaling pathway;>>Th17 cell differentiation;>>Thermogenesis;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Growth hormone synthesis, secretion and action;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>He
    • Gene Name：
    • MTOR
    • Protein Name：
    • Serine/threonine-protein kinase mTOR
    • Human Gene Id：
    • 2475
    • Human Swiss Prot No：
    • P42345
    • Mouse Swiss Prot No：
    • Q9JLN9
    • Immunogen：
    • The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448. AA range:2415-2464
    • Specificity：
    • Phospho-mTOR (S2448) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at S2448.
    • Formulation：
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source：
    • Polyclonal, Rabbit,IgG
    • Dilution：
    • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
    • Purification：
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration：
    • 1 mg/ml
    • Storage Stability：
    • -15°C to -25°C/1 year(Do not lower than -25°C)
    • Other Name：
    • MTOR;FRAP;FRAP1;FRAP2;RAFT1;RAPT1;Serine/threonine-protein kinase mTOR;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic tar
    • Observed Band(KD)：
    • 289kD
    • Background：
    • The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],
    • Function：
    • function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis.,
    • Subcellular Location：
    • Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .
    • Expression：
    • Expressed in numerous tissues, with highest levels in testis.

    Attributes

    • Code：
    • YP0176
    • Size
    • 50μL / 100μL / 200μL
    • Price$：
    • 190 / 340 / 600
    • Delivery：
    • 2024-11-27
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