• Target：
• mTOR

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - other;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Apelin signaling pathway;>>Neutrophil extracellular trap formation;>>JAK-STAT signaling pathway;>>Th17 cell differentiation;>>Thermogenesis;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Growth hormone synthesis, secretion and action;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>He

• Gene Name：
• MTOR

• Protein Name：
• Serine/threonine-protein kinase mTOR

• Human Gene Id：
• 2475

• Human Swiss Prot No：
• P42345

• Mouse Gene Id：
• 56717

• Mouse Swiss Prot No：
• Q9JLN9

• Rat Gene Id：
• 56718

• Rat Swiss Prot No：
• P42346

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448. AA range:2415-2464

• Specificity：
• Phospho-mTOR (S2448) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at S2448.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MTOR;FRAP;FRAP1;FRAP2;RAFT1;RAPT1;Serine/threonine-protein kinase mTOR;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic tar

• Observed Band(KD)：
• 289kD

• Background：
• The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],

• Function：
• function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis.,

• Subcellular Location：
• Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .

• Expression：
• Expressed in numerous tissues, with highest levels in testis.

CFNC, a neocryptolepine derivative, inhibited the growth of gastric cancer AGS cells by inhibiting PI3K/AKT signaling pathway

Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy Molecular Medicine Reports Bing Jiang, Longfei Feng, Tao Yang, Wenjing Guo, Yangyang Li, Tao Wang, Chengguang Liu, Haixiang Su WB Human 97H cell

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop WB Human 1:1000 /SW620, HCT116 Cells

Synthesis and anticancer evaluations of novel 1H-imidazole [4,5-f][1,10] phenanthroline derivative for the treatment of colorectal cancer WB Human /HCT116, SW480 cells

Bacillus subtilis Produces Amino Acids to Stimulate Protein Synthesis in Ruminal Tissue Explants via the Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta–Serine/Threonine Kinase–Mammalian Target of Rapamycin Complex 1 Pathway WB Bovine 1:2000 rumen explants/

Dihydroartemisinin Triggers Ferroptosis in Multidrug-Resistant Leukemia Cells WB Human 1:1000 /K562 cell

Network Pharmacology-Based Strategy to Identify the Pharmacological Mechanisms of Pulsatilla Decoction against Crohn's Disease.. Frontiers in Pharmacology2022 Apr;13:844685-844685. Human,Mouse 1:1000 colon tissues

Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway Food Chem Toxicol. 2022 May;163:112909. WB Mouse kidney tissue

Epigenetic regulation of autophagy by the methyltransferase EZH2 through an MTOR-dependent pathway. Autophagy Autophagy. 2015;11(12):2309-2322 WB Human MCF-7 cell,HeLa cell,HCT116 cell

3-Epipachysamine B suppresses proliferation and induces apoptosis of breast cancer cell via PI3K/AKT/mTOR signaling pathway. LIFE SCIENCES Life Sci. 2021 Nov;285:119995 WB Human 1:1000 MCF-7 cell,MDA-MB-231 cell

Rapamycin Antagonizes BCRP-Mediated Drug Resistance Through the PI3K/Akt/mTOR Signaling Pathway in mPRα-Positive Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Apr;0:352 WB Human 1 : 500 MDA-MB-231/BCRP cell，MDA-MB-453/BCRP cell

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways. Scientific Reports 2015 Jul 24 WB Human 5-8F-shFlot-2 cell

Ganoderic acid improves 5-fluorouracil-induced cognitive dysfunction in mice. Food & Function Food Funct. 2021 Nov;: WB Mouse 1:1000 Hippocampus

p53 Mediates Colistin-Induced Autophagy and Apoptosis in PC-12 Cells. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Antimicrob Agents Ch. 2016 Sep;60(9):5294-5301 WB Rat 1:1000 PC-12 cell

Targeting glycometabolic reprogramming to restore the sensitivity of leukemia drug-resistant K562/ADM cells to adriamycin. LIFE SCIENCES Life Sci. 2018 Dec;215:1 WB Human 1:1000 K562 cell

d-Glucose and amino acid deficiency inhibits casein synthesis through JAK2/STAT5 and AMPK/mTOR signaling pathways in mammary epithelial cells of dairy cows. JOURNAL OF DAIRY SCIENCE J Dairy Sci. 2018 Feb;101:1737 WB Bovine cow mammary epithelial cells (CMEC)

RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1. Cancer Medicine Cancer Med-Us. 2020 Feb;9(4):1529-1543 WB Human 1:2000 RKO cell, SW480 cell, HCT116 cell,LoVo cell

Anti-apoptotic and apoptotic pathway analysis of arsenic trioxide‑induced apoptosis in human gastric cancer SGC-7901 cells. ONCOLOGY REPORTS 2014 Jun 20 WB Human 1:800 SGC-7901 cell

PM2.5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells. International Journal of Biological Sciences Int J Biol Sci. 2018; 14(5): 557–564 WB Human BEASE-2B cell

Outer membrane protein a in Acinetobacter baumannii induces autophagy through mTOR signalling pathways in the lung of SD rats. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2021 Mar;135:111034 IF,IHC,WB Rat 1:100,1: 500,1:100 Lung WTRL1 cell

Uhrf1 regulates H3K9me2 modification of mTOR to inhibit the effect of autophagy in myocardial ischemia-reperfusion injury. Aging-US Aging-Us. 2021 Apr 15; 13(7): 9704–9718 WB Mouse Cardiomyocytes

Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway. LIFE SCIENCES 2018 Dec 03 WB Mouse 1:1000 ovary tissues

Luteolin induces apoptosis in vitro through suppressing the MAPK and PI3K signaling pathways in gastric cancer. Oncology Letters Oncol Lett. 2017 Aug;14(2):1993-2000 WB Human 1:1000 BGC-823 cell

The role and regulatory mechanism of autophagy in hippocampal nerve cells of piglet damaged by deoxynivalenol. TOXICOLOGY IN VITRO Toxicol In Vitro. 2020 Aug;66:104837 WB Pig 1:1000 piglet hippocampal nerve cells (PHNCs)

G-CSF attenuates neuroinflammation and neuronal apoptosis via the mTOR/p70SK6 signaling pathway in neonatal Hypoxia-Ischemia rat model. BRAIN RESEARCH 2020 Apr 01 WB Rat 1:1000 brain

CDKL3 promotes osteosarcoma progression by activating Akt/PKB. Life Science Alliance 2020 May;3(5):e202000648 WB Human OS tissue U2OS cell

Insulin Attenuates Beta-Amyloid-Associated Insulin/Akt/EAAT Signaling Perturbations in Human Astrocytes. CELLULAR AND MOLECULAR NEUROBIOLOGY Cell Mol Neurobiol. 2016 Aug;36(6):851-864 WB Human 1:5000 Astrocytes

Methylation-induced silencing of miR-34a enhances chemoresistance by directly upregulating ATG4B-induced autophagy through AMPK/mTOR pathway in prostate cancer. ONCOLOGY REPORTS 2015 Oct 16 WB Human PC-3 cell, DU145 cell

Water‑soluble nano‑pearl powder promotes MC3T3‑E1 cell differentiation by enhancing autophagy via the MEK/ERK signaling pathway. Molecular Medicine Reports Mol Med Rep. 2018 Jul;18(1):993-1000 WB Mouse 1:200 MC3T3-E1 cell

Glycometabolic adaptation mediates the insensitivity of drug-resistant K562/ADM leukaemia cells to adriamycin via the AKT-mTOR/c-Myc signalling pathway. Molecular Medicine Reports Mol Med Rep. 2017 Apr;15(4):1869-1876 WB Human 1:1000 K562/ADM cell

Peroxisome proliferator‑activated receptor‑γ agonist inhibits the mammalian target of rapamycin signaling pathway and has a protective effect in a rat model of status epilepticus. Molecular Medicine Reports Mol Med Rep. 2015 Aug;12(2):1877-1883 WB,IHC Rat 1:65,1:500 hippocampal tissues

Lu, Xueying, et al. "Luteolin induces apoptosis in vitro through suppressing the MAPK and PI3K signaling pathways in gastric cancer." Oncology letters 14.2 (2017): 1993-2000.

Xing, Yang, and Dan Zou. "Ethanol-induced cognitive dysfunction is associated with alterations in the mammalian target of rapamycin signalling pathway in the hippocampus of male mice." NeuroReport 29.14 (2018): 1230-1237.

Wang, F., et al. "Relationship between lysine/methionine ratios and glucose levels and their effects on casein synthesis via activation of the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells." Journal of dairy science 102.9 (2019): 8127-8133.

6-Methoxydihydrosanguinarine induces apoptosis and autophagy in breast cancer MCF-7 cells by accumulating ROS to suppress the PI3K/AKT/mTOR signaling pathway PHYTOTHERAPY RESEARCH Hui Zhang WB Human

The natural medicinal fungus Huaier promotes the anti-hepatoma efficacy of sorafenib through the mammalian target of rapamycin-mediated autophagic cell death MEDICAL ONCOLOGY Zhengguang Zhang WB Mouse, Human

UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer. Jing Wang WB Human 1:1000 ovarian tissue A2780 cell,OVCAR8 cell

ZEA mediates autophagy through the ROS-AMPK-m-TOR pathway to enhance the susceptibility of mastitis induced by Staphylococcus aureus in mice. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Xiaoyu Hu WB Mouse mouse mammary epithelial cells (MMEC)

Crosstalk between autophagy inhibitor and salidroside-induced apoptosis: A novel strategy for autophagy-based treatment of hepatocellular cancer. INTERNATIONAL IMMUNOPHARMACOLOGY Haixiang Su WB Human 1:1000 HepG2 cell,97H cell

Jiangqi Pingxiao formula regulates dendritic cell apoptosis in an autophagy-dependent manner through the AMPK/mTOR pathway in a murine model of OVA-induced asthma JOURNAL OF ETHNOPHARMACOLOGY Yiwen Shan WB Mouse 1:1000 bone marrow dendritic cells (BMDC)

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