• Target：
• JAK2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Cholinergic synapse;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Leishmaniasis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis

• Gene Name：
• JAK2

• Protein Name：
• Tyrosine-protein kinase JAK2

• Human Gene Id：
• 3717

• Human Swiss Prot No：
• O60674

• Mouse Gene Id：
• 16452

• Mouse Swiss Prot No：
• Q62120

• Rat Gene Id：
• 24514

• Rat Swiss Prot No：
• Q62689

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030

• Specificity：
• Phospho-JAK2 (Y1007) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y1007.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• JAK2;Tyrosine-protein kinase JAK2;Janus kinase 2;JAK-2

• Observed Band(KD)：
• 130kD

• Background：
• This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.,disease:Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.,disease:Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients incl

• Subcellular Location：
• Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .

• Expression：
• Ubiquitously expressed throughout most tissues.

HIF‐1α facilitates osteocyte‐mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro. JOURNAL OF CELLULAR PHYSIOLOGY 2019 Apr 29 WB Mouse 1:500 MLO-Y4 cell

A novel STAT3 inhibitor negatively modulates platelet activation and aggregation. ACTA PHARMACOLOGICA SINICA 2017 Mar 06 WB Human Platelets

Angiotensin II enhances the acetylation and release of HMGB1 in RAW264.7 macrophage. CELL BIOLOGY INTERNATIONAL 2018 Jul 23 WB Mouse RAW 264.7 cell

Deregulation of PRDM5 promotes cell proliferation by regulating JAK/STAT signaling pathway through SOCS1 in human lung adenocarcinoma Cancer Medicine Xin Liu WB Human

Cadmium contributes to atherosclerosis by affecting macrophage polarization FOOD AND CHEMICAL TOXICOLOGY Jia Song IHC,WB Mouse carotid RAW264.7 cells

Modified Erchen decoction ameliorates cognitive dysfunction in vascular dementia rats via inhibiting JAK2/STAT3 and JNK/BAX signaling pathways PHYTOMEDICINE Menghua Liu IHC Rat brain,liver

A hybrid nanopharmaceutical for specific-amplifying oxidative stress to initiate a cascade of catalytic therapy for pancreatic cancer. Yang Sun WB,IF Mouse 1:1000 PANC-1 cell-Xenograft PANC-1 cell

Buqi Huoxue Tongnao prescription protects against chronic cerebral hypoperfusion via regulating PI3K/AKT and LXRα/CYP7A1 signaling pathways PHYTOMEDICINE Yinhuang Gao WB 1:5000

JAK2 inhibitor protects the septic heart through enhancing mitophagy in cardiomyocytes BIOMEDICINE & PHARMACOTHERAPY Dafei Han WB Mouse,Human heart tissue AC16 cell

miR-135b-5p promotes gastric carcinogenesis by targeting CLIP4-mediated JAK2/STAT3 signal pathway CELLULAR SIGNALLING Zhiwei Peng WB Human 1:750 HGC-27 cell,AGS cell

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