GSK3α/β (phospho Tyr279/216) Polyclonal Antibody

    • Catalog No.:YP0123
    • Applications:IF;WB;IHC;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • GSK3α/β
      • Fields:
      • >>Chemokine signaling pathway;>>Dopaminergic synapse;>>Non-alcoholic fatty liver disease;>>Shigellosis
      • Gene Name:
      • GSK3A/GSK3B
      • Protein Name:
      • Glycogen synthase kinase-3 alpha/beta
      • Human Gene Id:
      • 2931
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295
      • Specificity:
      • Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GSK3A;Glycogen synthase kinase-3 alpha;GSK-3 alpha;Serine/threonine-protein kinase GSK3A;GSK3B;Glycogen synthase kinase-3 beta;GSK-3 beta;Serine/threonine-protein kinase GSK3B
      • Observed Band(KD):
      • 51,46kD
      • Background:
      • glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],
      • Function:
      • catalytic activity:ATP + [tau protein] = ADP + [tau protein] phosphate.,function:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer.,
      • Subcellular Location:
      • mitochondrion,cytosol,beta-catenin destruction complex,postsynapse,
      • Expression:
      • Brain,Eye,Foreskin,Lung,Pancreas,Platelet,Testis,
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
      • Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of various cells using Phospho-GSK3α/β (Y279/216) Polyclonal Antibody
      • Western Blot analysis of KB cells using Phospho-GSK3α/β (Y279/216) Polyclonal Antibody
      • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.