• Target：
• ERK 1/2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>TGF-beta signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Adherens junction;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>C-type lectin recep

• Gene Name：
• MAPK1/MAPK3

• Protein Name：
• Mitogen-activated protein kinase 3

• Human Gene Id：
• 5595/5594

• Human Swiss Prot No：
• P27361/P28482

• Mouse Gene Id：
• 26417/26413

• Rat Gene Id：
• 50689/116590

• Rat Swiss Prot No：
• P21708/P63086

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Tyr204. AA range:170-219

• Specificity：
• Phospho-ERK 1/2 (Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y204.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK3;ERK1;PRKM3;Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p

• Observed Band(KD)：
• 44+42kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF.,function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,PTM:Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinas

• Subcellular Location：
• Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .

• Expression：
• Epithelium,Eye,Hepatoma,Human cervix,Lymph,

GCN5 participates in KLF4-VEGFA feedback to promote endometrial angiogenesis WB Human /HEMECs

Chamaejasmenin E from Stellera chamaejasme induces apoptosis of hepatocellular carcinoma cells by targeting c-Met in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2022 Feb;119:105509 WB Human 1:800 Hep3B cell，SMMC-7721 cell

Matrine inhibits BCR/ABL mediated ERK/MAPK pathway in human leukemia cells. Oncotarget Oncotarget. 2017 Dec 12; 8(65): 108880–108889 WB Human 1:500 ,1:2000 K562 cell, HL-60 cell

Amelioration of diabetic retinopathy in db/db mice by treatment with different proportional three active ingredients from Tibetan medicine Berberis dictyophylla F. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2021 Aug;276:114190 IF Mouse 1 : 100 Retina

LASP2 is downregulated in human liver cancer and contributes to hepatoblastoma cell malignant phenotypes through MAPK/ERK pathway. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Jul;127:110154 WB Human 1:1000 HepG2 cell

Effects of hydroxysafflor yellow A on rats with collagen-induced arthritis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2021 Sep;570:26 WB Rat 1:500 Synovial

Crassifoside H improve the depressive-like behavior of rats under chronic unpredictable mild stress: Possible involved mechanisms. BRAIN RESEARCH BULLETIN Brain Res Bull. 2017 Oct;135:77 WB Rat 1:1000 hippocampus , hypothalamus

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib. ONCOLOGY REPORTS 2014 Dec 11 WB Human MDA-MB-468 cell

Liu, Zhiyu, et al. "Calcium signaling and the MAPK cascade are required for sperm activation in Caenorhabditis elegans." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1843.2 (2014): 299-308.

Ma, Yucong, et al. "Morroniside protect human granulosa cells against H2O2-induced oxidative damage via regulating Nrf2 and MAPK signaling pathway." (2021).

Qingjie, K. A. N. G., and X. I. A. N. G. Zheng. "Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620." China Oncology 30.10 (2020): 785-790.

Meng, Xiangli, et al. "Integrin beta 4 (ITGB4) and its tyrosine-1510 phosphorylation promote pancreatic tumorigenesis and regulate the MEK1-ERK1/2 signaling pathway." Bosnian journal of basic medical sciences 20.1 (2020): 106.

康清杰, and 向征. "沉默胶质瘤相关癌基因同源物 2 基因对结肠癌细胞系 SW620 增殖和凋亡的影响." 中国癌症杂志 30.10 (2020): 785-790.

The analgesic effects of β-elemene in rats with neuropathic pain by inhibition of spinal astrocytic ERK activation: Molecular Pain Jin Zheng WB Rat

Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota PHYTOMEDICINE Shaohui Wang WB Rat

Morroniside Protects Human Granulosa Cells against H₂O₂-Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways Evidence-based Complementary and Alternative Medicine Huilan Du WB Human

Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway PLATELETS Lihua Yin WB Human Human bone marrow stem cell (hBMSCs)

HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell,Huh7 cell

Silencing LCN2 suppresses oral squamous cell carcinoma progression by reducing EGFR signal activation and recycling JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Zhiquan Huang WB Human CAL-27ER cell,HN-6ER cell,CAL-27 cell,HN-6 cell

FGF12 regulates cell cycle gene expression and promotes follicular granulosa cell proliferation through ERK phosphorylation in geese. Xingyong Chen WB Geese 1:1000 granulosa cell

mGluR1/IP3/ERK signaling pathway regulates vestibular compensation in ON UBCs of the cerebellar flocculus. CNS Neuroscience & Therapeutics Sulin Zhang WB Rat 1:1000 Flocculus tissue

The Effects of Injectable Platelet-Rich Fibrin and Advanced-Platelet Rich Fibrin on Gingival Fibroblast Cell Vitality, Proliferation, Differentiation. Tissue Engineering and Regenerative Medicine Yin Lihua WB Human 1:1000 human gingival fibroblast cells (HGFCs)

Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Banfeng Ruan WB Mouse 1:1000 RAW264.7 cell

Design, synthesis, in vitro and in vivo biological evaluation of pterostilbene derivatives for anti-inflammation therapy JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Quanren Cui WB Mouse 1:1000 RAW264.7 cell

The gut microbiota contributes to the infection of bovine viral diarrhea virus in mice JOURNAL OF VIROLOGY Zhanbo Zhu WB Mouse peripheral blood lymphocytes (PBL)

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