• Target：
• CaMKIV

• Fields：
• >>Calcium signaling pathway;>>cAMP signaling pathway;>>Longevity regulating pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Amphetamine addiction;>>Alcoholism;>>Glioma

• Gene Name：
• CAMK4

• Protein Name：
• Calcium/calmodulin-dependent protein kinase type IV

• Human Gene Id：
• 814

• Human Swiss Prot No：
• Q16566

• Mouse Swiss Prot No：
• P08414

• Rat Gene Id：
• 25050

• Rat Swiss Prot No：
• P13234

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215

• Specificity：
• Phospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at T200.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CAMK4;CAMK;CAMK-GR;CAMKIV;Calcium/calmodulin-dependent protein kinase type IV;CaMK IV;CaM kinase-GR

• Observed Band(KD)：
• 60kD

• Background：
• The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role i

• Subcellular Location：
• Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity). .

• Expression：
• Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.

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