AMPKα2 Polyclonal Antibody
- Catalog No.:YN5680
- Applications:WB
- Reactivity:Human;Mouse;Rat
- Target:
- AMPKα2
- Fields:
- >>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Apelin signaling pathway;>>Tight junction;>>Circadian rhythm;>>Thermogenesis;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hypertrophic cardiomyopathy;>>Fluid shear stress and atherosclerosis
- Gene Name:
- PRKAA2
- Protein Name:
- 5'-AMP-activated protein kinase catalytic subunit alpha-2
- Human Gene Id:
- 5563
- Human Swiss Prot No:
- P54646
- Mouse Swiss Prot No:
- Q8BRK8
- Rat Swiss Prot No:
- Q09137
- Immunogen:
- Recombinant Protein of AMPKα2
- Specificity:
- The antibody detects endogenous AMPKα2 protein.
- Formulation:
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:1000-2000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- PRKAA2;AMPK;AMPK2;5'-AMP-activated protein kinase catalytic subunit alpha-2;AMPK subunit alpha-2;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase
- Observed Band(KD):
- 62kD
- Background:
- The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic s
- Subcellular Location:
- Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
- Expression:
- Heart,Skeletal muscle,
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- Antibody-FAQs
- Products Images
- Western blot analysis of 1) Hela, 2) 293T, 3) C2C12, 4) 3T3, 5) Rat Heart, 6) Rat Brain using AMPKα2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000