• Target：
• LC3B

• Fields：
• >>Mitophagy - animal;>>Autophagy - animal;>>Ferroptosis;>>Apelin signaling pathway;>>NOD-like receptor signaling pathway;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection

• Gene Name：
• MAP1LC3B

• Protein Name：
• Microtubule-associated proteins 1A/1B light chain 3B

• Human Gene Id：
• 81631

• Human Swiss Prot No：
• Q9GZQ8

• Mouse Swiss Prot No：
• Q9CQV6

• Rat Swiss Prot No：
• Q62625

• Immunogen：
• Recombinant Protein of MAP LC3β

• Specificity：
• The antibody detects endogenous MAP LC3β protein.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:1000-2000 IHC 1:200-500 IF 1:200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAP1LC3B;MAP1ALC3;Microtubule-associated proteins 1A/1B light chain 3B;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B light chain 3 B;MAP1A/MAP1B LC3 B;Microtubule-associated protein 1 light chain 3 beta

• Observed Band(KD)：
• 14,16kD

• Background：
• The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],

• Function：
• caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,

• Subcellular Location：
• Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .

• Expression：
• Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

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