• Target：
• ATG5

• Fields：
• >>Autophagy - other;>>Mitophagy - animal;>>Autophagy - animal;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Ferroptosis;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Shigellosis

• Gene Name：
• ATG5 APG5L ASP

• Protein Name：
• ATG5

• Human Gene Id：
• 9474

• Human Swiss Prot No：
• Q9H1Y0

• Mouse Gene Id：
• 11793

• Mouse Swiss Prot No：
• Q99J83

• Rat Swiss Prot No：
• Q3MQ06

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Autophagy protein 5 (APG5-like;Apoptosis-specific protein)

• Molecular Weight(Da)：
• 33kD

• Observed Band(KD)：
• 55kD

• Background：
• The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],

• Function：
• function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.,

• Subcellular Location：
• Cytoplasm

• Expression：
• Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Ammonia inhalation-mediated mir-202-5p leads to cardiac autophagy through PTEN/AKT/mTOR pathway. CHEMOSPHERE Chemosphere. 2019 Nov;235:858 WB Chicken 1:500 heart

PTH1-34 promotes osteoblast formation through Beclin1-dependent autophagic activation. JOURNAL OF BONE AND MINERAL METABOLISM J Bone Miner Metab. 2021 Jul;39(4):572-582 WB Mouse MC3T3-E1 cell

Fasting Therapy Contributes to the Improvement of Endothelial Function and Decline in Vascular Injury-Related Markers in Overweight and Obese Individuals via Activating Autophagy of Endothelial Progenitor Cells. Evidence-based Complementary and Alternative Medicine 2020 Jul 27 WB Mouse 1 : 1000 Endothelial progenitor cells

The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of Aβ and Autophagy. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2020;2020:6807879 IHC Rat 1 : 500 brain

Ammonia inhalation-mediated mir-202-5p leads to cardiac autophagy through PTEN/AKT/mTOR pathway. CHEMOSPHERE Chemosphere. 2019 Nov;235:858 WB Chicken 1:500 heart

PTH1-34 promotes osteoblast formation through Beclin1-dependent autophagic activation. JOURNAL OF BONE AND MINERAL METABOLISM J Bone Miner Metab. 2021 Jul;39(4):572-582 WB Mouse MC3T3-E1 cell

Fasting Therapy Contributes to the Improvement of Endothelial Function and Decline in Vascular Injury-Related Markers in Overweight and Obese Individuals via Activating Autophagy of Endothelial Progenitor Cells. Evidence-based Complementary and Alternative Medicine 2020 Jul 27 WB Mouse 1 : 1000 Endothelial progenitor cells

The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of Aβ and Autophagy. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2020;2020:6807879 IHC Rat 1 : 500 brain

Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition. Qiang Yuan IHC Mouse 1:100 H1299 cell-Xenograft

Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy Antioxidants Zixuan Yuan WB Mouse 1:1000 brain tissue HT22 cell

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