MEK1/2 (PT0433R) PT® Rabbit mAb

    • Catalog No.:YM8273
    • Applications:WB;IHC;IF;IP;ELISA
    • Reactivity:Human; Mouse; Rat;
      • Target:
      • MEK-1/2
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Cellular senescence;>>Vascular smooth muscle contraction;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>TNF signaling pathway;>>Lon
      • Gene Name:
      • MAP2K1/MAP2K2
      • Protein Name:
      • Dual specificity mitogen-activated protein kinase kinase 1/2
      • Specificity:
      • endogenous
      • Formulation:
      • PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
      • Source:
      • Monoclonal, rabbit, IgG, Kappa
      • Dilution:
      • IHC 1:500-1:2000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
      • Purification:
      • Protein A
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • MAP2K1;MEK1;PRKMK1;Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K2;MEK2;MKK2;PRKMK2;Dual specificity mitogen-activated protein k
      • Molecular Weight(Da):
      • 44kD
      • Observed Band(KD):
      • 44kD
      • Background:
      • The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,enzyme reg
      • Subcellular Location:
      • Cytoplasm
      • Expression:
      • Widely expressed, with extremely low levels in brain.
      • Products Images
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- MEK1/2 (PT0433R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: C6 Lane 3: 3T3-L1 Lane 4: K562 Predicted band size: 44kDa Observed band size: 44kDa
      • Human lung carcinoma was stained with anti-MEK1/2 (PT0433R) rabbit antibody
      • Mouse colon was stained with anti-MEK1/2 (PT0433R) rabbit antibody