• Target：
• DRP1

• Fields：
• >>Necroptosis;>>NOD-like receptor signaling pathway;>>TNF signaling pathway

• Gene Name：
• DNM1L

• Protein Name：
• Dynamin-1-like protein

• Human Gene Id：
• 10059

• Human Swiss Prot No：
• O00429

• Mouse Gene Id：
• 74006

• Mouse Swiss Prot No：
• Q8K1M6

• Rat Gene Id：
• 114114

• Rat Swiss Prot No：
• O35303

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:200-1000,WB 1:1000-5000,IF 1:200-1000,ELISA 1:5000-20000,IP 1:50-200

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• DNM1L;DLP1;DRP1;Dynamin-1-like protein;Dnm1p/Vps1p-like protein;DVLP;Dynamin family member proline-rich carboxyl-terminal domain less;Dymple;Dynamin-like protein;Dynamin-like protein 4;Dynamin-like protein IV;HdynIV;Dynamin-rela

• Molecular Weight(Da)：
• 83kD

• Observed Band(KD)：
• 83kD

• Background：
• This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013],

• Function：
• catalytic activity:GTP + H(2)O = GDP + phosphate.,function:Functions in mitochondrial and peroxisomal division probably by regulating membrane fission. Enzyme hydrolyzing GTP that oligomerizes to form ring-like structures and is able to remodel membranes. May also play a role on organelles of the secretory pathway.,miscellaneous:Isoform 1 and isoform 2 inhibits peroxisomal division when overexpressed while isoform 3 and isoform 4 have no effect.,PTM:Phosphorylated by GSK3B.,similarity:Belongs to the dynamin family.,similarity:Contains 1 GED domain.,subcellular location:Mainly cytosolic. Also membrane-associated. Localizes to mitochondria at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear.,subunit:Homotetramer; N-terminal part b

• Subcellular Location：
• Cytoplasm

• Expression：
• Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.

Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia. Experimental and Therapeutic Medicine 2019 Mar 01 WB Human,Rat 1:1000 lung WRTL1 cell

Experimental and therapeutic medicine 17.3 (2019): 2349-2358.

miR-34a/DRP-1-mediated mitophagy participated in cisplatin-induced ototoxicity via increasing oxidative stress BMC Pharmacology & Toxicology Haidi Yang WB Mouse cochlear tissue HEI-OC1 cell

PIM1 attenuates cisplatin-induced AKI by inhibiting Drp1 activation CELLULAR SIGNALLING Yuzhen Li WB Mouse 1:1000 kidney tissue mouse proximal tubular cell

ALCAT1-mediated abnormal cardiolipin remodelling promotes mitochondrial injury in podocytes in diabetic kidney disease Cell Communication and Signaling Hao Yiqun WB Human,Mouse 1:1000 glomerulus tissue podocytes

Human umbilical cord-derived mesenchymal stem cells ameliorate liver fibrosis by improving mitochondrial function via Slc25a47-Sirt3 signaling pathway BIOMEDICINE & PHARMACOTHERAPY Ping Chen IF Mouse liver tissue

Non-cytopathic bovine viral diarrhea virus (BVDV) inhibits innate immune responses via induction of mitophagy VETERINARY RESEARCH Li Zhijun WB Bovine MDBK cell

Gui Qi Zhuang Jin Decoction ameliorates mitochondrial dysfunction in sarcopenia mice via AMPK/PGC-1α/Nrf2 axis revealed by a metabolomics approach PHYTOMEDICINE Dong Wang IF Mouse gastrocnemius muscle tissue

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