Neuron-Specific Enolase(NSE) (ABT47R) rabbit mAb
- Catalog No.：YM7270
- Applications：IHC;WB; ELISA
- Reactivity：Human; Mouse; (predicted: Rat)
- Target：
- Enolase
- Fields：
- >>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>RNA degradation;>>HIF-1 signaling pathway
- Gene Name：
- ENO2
- Protein Name：
- Neuron-Specific Enolase(NSE)
- Human Gene Id：
- 2026
- Human Swiss Prot No：
- P09104
- Immunogen：
- Synthesized peptide derived from human Neuron-Specific Enolase(NSE) AA range:300-434
- Specificity：
- This antibody detects endogenous levels of Enolase
- Formulation：
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source：
- Monoclonal, Rabbit IgG1, Kappa
- Dilution：
- IHC 1:100-500, WB 1:500-1000, ELISA 1:5000-20000
- Purification：
- Recombinant Expression and Affinity purified
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- 2 phospho D glyceRate hydrolyase;2-phospho-D-glyceRate hydro-lyase;Eno 2;ENO2;ENOG;ENOG_HUMAN;Enolase 2 (gamma, neuronal);Enolase 2;Enolase 2 gamma neuronal;Enolase2;Epididymis secretory protein Li 279;Gamma enolase;Gamma-enolase;HEL S 279;Neural enolase;Neuron specific enolase;Neuron specific gamma enolase;Neuron-specific enolase;neuronal enriched enolase;Neurone specific enolase;NSE
- Molecular Weight(Da)：
- 47kD
- Background：
- enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],
- Function：
- catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane
- Subcellular Location：
- Cytoplasmic
- Expression：
- The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human Colon. 1, Antibody was incubated at 4° overnight. 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunohistochemical analysis of paraffin-embedded human Pancreas. 1, Antibody was incubated at 4° overnight. 2, TRIS-EDTA of pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
