Cytokeratin 8&18 (ABT053/CK18) Mouse mAb

    • Catalog No.:YM6995
    • Applications:IHC;WB;ELISA
    • Reactivity:Human; Mouse (predicted: Rat)
      • Target:
      • Cytokeratin 8/18
      • Gene Name:
      • KRT8/KRT18
      • Immunogen:
      • Synthesized peptide derived from human Cytokeratin 8&18 AA range: C-term to CK8 and 200-300 to CK18
      • Specificity:
      • This product can specifically recognize human CK8 and CK18 protein. It is a cocktail of anti-CK8 and anti-CK18 monoclonal antibodies.
      • Formulation:
      • PBS, pH7.2, 0.03% Porcolin 300, containing stabilizing protein
      • Source:
      • Monoclonal Mouse IgG2b, Kappa
      • Dilution:
      • IHC-p 1:200-400,WB 500-1000, ELISA 1:5000-20000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Molecular Weight(Da):
      • 53kD
      • Background:
      • keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
      • Function:
      • disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue spec
      • Subcellular Location:
      • Cytoplasmic, Membranous
      • Expression:
      • Liver/ Tonsil
      • Products Images
      • Human colon carcinoma tissue was stained with Anti-Cytokeratin 8/18 (ABT053/ABT-CK18) Antibody
      • Human gastric adenocarcinoma tissue was stained with Anti-Cytokeratin 8/18 (ABT053/ABT-CK18) Antibody
      • Human kidney tissue was stained with Anti-Cytokeratin 8/18 (ABT053/ABT-CK18) Antibody
      • Human liver tissue was stained with Anti-Cytokeratin 8/18 (ABT053/ABT-CK18) Antibody
      • Human lung adenocarcinoma tissue was stained with Anti-Cytokeratin 8/18 (ABT053/ABT-CK18) Antibody
      • Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8/18(ABT053/CK18)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT116 Lane 2: SW480 Lane 3: HepG2 Predicted band size: 48,54kDa Observed band size: 48,54kDa