Cytokeratin 19&10 (ABT-CK19) mouse mAb

    • Catalog No.:YM6577
    • Applications:IHC;WB;ELISA
    • Reactivity:Human;
      • Target:
      • Cytokeratin 19/10
      • Fields:
      • >>Estrogen signaling pathway;>>Staphylococcus aureus infection
      • Gene Name:
      • KRT19 KRT10
      • Protein Name:
      • Keratin, type I cytoskeletal 19/10 (Cytokeratin-19/10) (CK-19/10) (Keratin-19/10) (K19/10)
      • Human Gene Id:
      • 3880
      • Immunogen:
      • Synthesized peptide derived from human Cytokeratin 19 AA range: 200-300
      • Specificity:
      • This antibody detects endogenous levels of human Cytokeratin 19. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse, Monoclonal/IgG1, Kappa
      • Dilution:
      • IHC-p 1:200-400,WB 1:500-2000, ELISA 1:5000-20000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Background:
      • The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],
      • Function:
      • developmental stage:Present in hair follicles at all stages of development.,domain:This keratin differs from all other IF proteins in lacking the C-terminal tail domain.,function:Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.,tissue specificity:Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestin
      • Subcellular Location:
      • Cytoplasmic, Membranous
      • Expression:
      • Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
      • Products Images
      • Whole cell lysates of HCT116 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 19 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Predicted band size: 44 kDa
      • Human kidney tissue was stained with Anti-Cytokeratin 19&10 (ABT-CK19) Antibody