Alpha-1-Fetoprotein(AFP) (PT0679) Mouse mAb
- Catalog No.：YM4089
- Applications：WB;IHC;ELISA
- Reactivity：Human; Mouse (predicted: Rat)
- Target：
- AFP
- Fields：
- >>Hippo signaling pathway
- Gene Name：
- AFP
- Protein Name：
- Alpha-1-Fetoprotein(AFP)
- Human Gene Id：
- 174
- Human Swiss Prot No：
- P02771
- Immunogen：
- Synthesized peptide derived from human Alpha-1-Fetoprotein(AFP) AA range: 350-450
- Specificity：
- This antibody detects endogenous levels of human Alpha-1-Fetoprotein(AFP)
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Monoclonal,Mouse IgG1, Kappa
- Dilution：
- WB 1:500-2000,IHC 1:100-300,ELISA 1:5000-20000
- Purification：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- Alpha-fetoprotein (Alpha-1-fetoprotein) (Alpha-fetoglobulin)
- Molecular Weight(Da)：
- 69kD
- Background：
- This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],
- Function：
- developmental stage:Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.,function:Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.,online information:Alpha-fetoprotein entry,PTM:Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.,PTM:Sulfated.,similarity:Belongs to the ALB/AFP/VDB family.,similarity:Contains 3 albumin domains.,subunit:Dimeric and trimeric forms have been found in addition to the monomeric form.,tissue
- Subcellular Location：
- Secreted.
- Expression：
- Plasma. Synthesized by the fetal liver and yolk sac.
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- Antibody-FAQs
- Products Images
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-AFP (PT0679) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HepG2 Lane 3: mouse liver Predicted band size: 69kDa Observed band size: 69kDa
