• Purity：
• >95% SDS-PAGE

• Target：
• Flag-tag

• Fields：
• This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.

• Gene Name：
• Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag

• Immunogen：
• Synthetic Peptide of Flag-Tag

• Specificity：
• The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Rabbit VH IgG1 and VL Kappa was expressed from 293F cells

• Dilution：
• WB 1:1000-5000 ELISA 1:20000-50000 IF 1:100-300

• Purification：
• Protein A purified

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Background：
• The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

Phosphorylation of PBK at Thr9 by CDK5 correlates with invasion of prolactinomas CNS Neuroscience & Therapeutics Qiuyue Fang WB,CoIP Mouse,Human 1:5000 GH3 cell-Xenograft HEK293T cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs