Bax mouse Monoclonal Antibody(6F11)

    • Catalog No.:YM3619
    • Applications:IF,WB,IHC-p
    • Reactivity:Human,Rat,Mouse,Chicken
      • Gene Name:
      • BAX
      • Protein Name:
      • BAX
      • Human Gene Id:
      • 581
      • Human Swiss Prot No:
      • Q07812
      • Mouse Swiss Prot No:
      • Q07813
      • Immunogen:
      • Synthetic Peptide of Bax at AA range of 30-110
      • Specificity:
      • Bax protein detects endogenous levels of BAX
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse
      • Dilution:
      • IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • BAX
      • Observed Band(KD):
      • 20
      • Background:
      • The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.
      • Function:
      • disease:Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.,domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.,similarity:Belongs to the Bcl-2 family.,subcellular location:Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, redistributes to the mitochondrion membrane through the release from JNK-phosphorylated 14-3-3 proteins.,subunit:Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1
      • Products Images
      • Western blot analysis of lysates from 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate cells, (Green) primary antibody was diluted at 1:1000, 4°over night,Dylight 800 secondary antibody(Immunoway:RS23910)was diluted at 1:10000, 37° 1hour. (Red) Actin β Polyclonal Antibody (Immunoway:YT0099) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23720)was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse mAb diluted at 1:200.
      • Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse mAb diluted at 1:200.
      • Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse mAb diluted at 1:200.
      • Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.
      • Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000