Bax mouse Monoclonal Antibody(6F11)

    • Catalog No.:YM3619
    • Applications:IF,WB,IHC-p
    • Reactivity:Human,Rat,Mouse,Chicken
    • Description
    • Gene Name:
    • BAX
    • Protein Name:
    • BAX
    • Human Gene Id:
    • 581
    • Human Swiss Prot No:
    • Q07812
    • Mouse Swiss Prot No:
    • Q07813
    • Immunogen:
    • Synthetic Peptide of Bax at AA range of 30-110
    • Specificity:
    • Bax protein detects endogenous levels of BAX
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Mouse
    • Dilution:
    • IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • BAX
    • Observed Band(KD):
    • 20
    • Background:
    • disease:Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.,domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.,similarity:Belongs to the Bcl-2 family.,subcellular location:Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, redistributes to the mitochondrion membrane through the release from JNK-phosphorylated 14-3-3 proteins.,subunit:Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L).,tissue specificity:Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histyocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.,
    • Function:
    • disease:Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.,domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.,similarity:Belongs to the Bcl-2 family.,subcellular location:Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, redistributes to the mitochondrion membrane through the release from JNK-phosphorylated 14-3-3 proteins.,subunit:Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse mAb diluted at 1:200.
    • Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse mAb diluted at 1:200.
    • Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse mAb diluted at 1:200.
    • Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.
    • Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000