• Target：
• Bax

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>Sphingolipid signaling pathway;>>p53 signaling pathway;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Longevity regulating pathway;>>Apoptosis - multiple species;>>Necroptosis;>>Neurotrophin signaling pathway;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Transcriptional

• Gene Name：
• BAX

• Protein Name：
• BAX

• Human Gene Id：
• 581

• Human Swiss Prot No：
• Q07812

• Immunogen：
• Synthesized peptide derived from human Bax AA range: 50-150

• Specificity：
• This antibody detects endogenous levels of bax.

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Mouse, Monoclonal/IgG1, kappa

• Dilution：
• WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000

• Purification：
• Protein G

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BAX;BCL2L4

• Molecular Weight(Da)：
• 21kD

• Observed Band(KD)：
• 21kD

• Background：
• The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

• Function：
• disease:Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.,domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.,similarity:Belongs to the Bcl-2 family.,subcellular location:Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, redistributes to the mitochondrion membrane through the release from JNK-phosphorylated 14-3-3 proteins.,subunit:Homodimer. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), MCL1

• Subcellular Location：
• Cytoplasmic

• Expression：
• Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics. Nature Communications Nat Commun. 2020 Oct;11(1):1-11 WB Human A549 cell

A preparation of Ginkgo biloba L. leaves extract inhibits the apoptosis of hippocampal neurons in post-stroke mice via regulating the expression of Bax/Bcl-2 and Caspase-3. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2021 Nov;280:114481 IHC,IF,WB Mouse 1:110,1:1000 Hippocampus Hippocampal cell line HT-22

The Effect of SPTLC2 on Promoting Neuronal Apoptosis is Alleviated by MiR-124-3p Through TLR4 Signalling Pathway. NEUROCHEMICAL RESEARCH Neurochem Res. 2019 Sep;44(9):2113-2122 WB Mouse 1:2000 cortical neurons

Emodin suppresses the migration and invasion of melanoma cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN 2021 Mar 17 WB Mouse,Human 1 : 500 B16F10 Melanoma Cells， A375 Melanoma Cells

HN1L is essential for cell growth and survival during nucleopolyhedrovirus infection in silkworm, Bombyx mori. PLoS One Plos One. 2019 May;14(5):e0216719 WB Silkworm 1:1000 BmN cell

Mesenchymal stem cell-derived extracellular vesicles promote apoptosis in RSC96 Schwann cells through the activation of the ERK pathway. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2018; 11(11): 5157–5170 WB Rat RSC96 cell

Suppression of Cellular Proliferation and Invasion by HMGB1 Knockdown in Bladder Urothelial Carcinoma Cells. ONCOLOGY RESEARCH Oncol Res. 2015; 22(5-6): 235–245 WB Human BIU-87 cell, T24 cell

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide. Analytical Cellular Pathology Anal Cell Pathol. 2021;2021:1781532 WB Mouse 1:1000 Ovarian tissue

Chlamydia psittaci inclusion membrane protein CPSIT_0842 induces macrophage apoptosis through MAPK/ERK-mediated autophagy INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY Yimou Wu WB Human human monocytic leukemia cell line THP-1 cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:2000 IEC-6 cell

L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells Journal of Functional Foods Min Long WB Rat 1:2000 L6 cell

Phillyrin reduces ROS production to alleviate the progression of intervertebral disc degeneration by inhibiting NF-κB pathway Journal of Orthopaedic Surgery and Research Chen Enming WB Rat 1:1000 nucleus pulposus cell (NPC)

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