AMPK α1 Monoclonal Antibody(5G11)

    • Catalog No.：YM3520
    • Applications：WB;IHC;IF
    • Reactivity：Human
      • Target：
      • AMPKα1
      • Fields：
      • >>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Apelin signaling pathway;>>Tight junction;>>Circadian rhythm;>>Thermogenesis;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hypertrophic cardiomyopathy;>>Fluid shear stress and atherosclerosis
      • Gene Name：
      • PRKAA1
      • Protein Name：
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.11.27) (Hydroxymethylglutaryl-CoA reductase kinase
      • Human Gene Id：
      • 5562
      • Human Swiss Prot No：
      • Q13131
      • Mouse Swiss Prot No：
      • Q5EG47
      • Immunogen：
      • Synthetic Peptide of AMPK α1
      • Specificity：
      • AMPK α1 protein detects endogenous levels of AMPK α1
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Monoclonal, Mouse
      • Dilution：
      • WB 1:1000-2000, IHC 1:50-100. IF 1:50-200
      • Purification：
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1
      • Observed Band(KD)：
      • 63kD
      • Background：
      • The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
      • Function：
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic s
      • Subcellular Location：
      • Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
      • Expression：
      • Brain,Intestine,Liver,Mammary gland,Platelet,Testis
      • Products Images
      • Western blot analysis of 1)Hela, 2) 293T, 3)3T3, 4) PC12 with AMPK a1 Mouse mAb diluted at 1:2,000.
      • Immunohistochemical analysis of paraffin-embedded Mouse ColonTissue using AMPK a1 Mouse mAb diluted at 1:200.

      Attributes

      • Code：
      • YM3520
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 160 / 280 / 450
      • Delivery：
      • 2024-11-25
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