HAO1 Monoclonal Antibody(Mix)

    • Catalog No.:YM3378
    • Applications:WB,IHC-p,IF,
    • Reactivity:Mouse,Rat
    • Description
    • Gene Name:
    • HAO1
    • Protein Name:
    • Hydroxyacid oxidase 1
    • Human Swiss Prot No:
    • Q9UJM8
    • Mouse Swiss Prot No:
    • Q9WU19
    • Immunogen:
    • Recombinant Protein of HAO1
    • Specificity:
    • The antibody detects endogenous HAO1 protein.
    • Formulation:
    • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    • Source:
    • Mouse
    • Dilution:
    • WB: 1:1000-2000 IF 1:200 IHC 1:50-300
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX
    • Observed Band(KD):
    • 41
    • Background:
    • HAO1 (hydroxyacid oxidase 1) is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, HAO1 is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
    • Function:
    • fatty acid alpha-oxidation, cellular aldehyde metabolic process, fatty acid metabolic process, fatty acid catabolic process, lipid catabolic process, organic acid catabolic process, fatty acid oxidation, lipid modification, lipid oxidation,cellular lipid catabolic process, carboxylic acid catabolic process, glyoxylate metabolic process, oxidation reduction,
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunofluorescence analysis of Human-appendix tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Immunofluorescence analysis of Mouse-spleen tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.