• Target：
• HAO1

• Fields：
• >>Glyoxylate and dicarboxylate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Peroxisome

• Gene Name：
• HAO1

• Protein Name：
• Hydroxyacid oxidase 1

• Human Gene Id：
• 54363

• Human Swiss Prot No：
• Q9UJM8

• Mouse Swiss Prot No：
• Q9WU19

• Immunogen：
• Recombinant Protein of HAO1

• Specificity：
• The antibody detects endogenous HAO1 protein.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:1000-2000 IF 1:200 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Hydroxyacid oxidase 1;HAOX1;Glycolate oxidase;GOX

• Observed Band(KD)：
• 41kD

• Background：
• This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,

• Subcellular Location：
• Peroxisome matrix .

• Expression：
• Highly expressed in liver.

HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps WB,IF Mouse 1:1000, 1:200 lung/alveolar epithelial cells

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