• Target：
• NOS3

• Fields：
• >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Platelet activation;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• NOS3

• Protein Name：
• Nitric oxide synthase, endothelial

• Human Gene Id：
• 4846

• Human Swiss Prot No：
• P29474

• Mouse Gene Id：
• 18127

• Mouse Swiss Prot No：
• P70313

• Rat Gene Id：
• 24600

• Rat Swiss Prot No：
• Q62600

• Immunogen：
• Recombinant Protein of eNOS

• Specificity：
• The antibody detects endogenous eNOS protein.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500-2000

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NOS3;Nitric oxide synthase, endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII

• Observed Band(KD)：
• 130-140kD

• Background：
• Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

• Function：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like

• Subcellular Location：
• Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

• Expression：
• Platelets, placenta, liver and kidney.

Honokiol improves endothelial function in type 2 diabetic rats via alleviating oxidative stress and insulin resistance Biochem Bioph Res Co. 2022 Feb;:. WB Human

Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels. Molecular Medicine Reports 2018 Sep 17 WB Rabbit 1:500 vascular tissues

TMAO Impairs Mouse Aortic Vasodilation by Inhibiting TRPV4 Channels in Endothelial Cells Journal of Cardiovascular Translational Research Zhang Ning WB Mouse 1:1000 aortic endothelial cell

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