• Target：
• Ki-67

• Gene Name：
• MKI67

• Protein Name：
• Ki 67

• Human Gene Id：
• 4288

• Human Swiss Prot No：
• P46013

• Immunogen：
• Synthetic Peptide of Ki 67

• Specificity：
• The antibody detects endogenous Ki 67 proteins.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• IHC 1:200 IF 1:50-200

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MKI67;Antigen KI-67

• Molecular Weight(Da)：
• 359kD

• Background：
• This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009],

• Function：
• developmental stage:Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.,function:Thought to be required for maintaining cell proliferation.,online information:Ki-67 entry,similarity:Contains 1 FHA domain.,subcellular location:Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.,subunit:Interacts with KIF15. Binds through the FHA domain to MKI67IP.,

• Subcellular Location：
• Chromosome . Nucleus . Nucleus, nucleolus . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). .

• Expression：
• Epithelium,

Activation of mTORC1 in subchondral bone preosteoblasts promotes osteoarthritis by stimulating bone sclerosis and secretion of CXCL12. Bone Research Bone Res. 2019 Feb;7(1):1-13 IHC Mouse Knee joint

EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization. Cell Death & Disease Cell Death Dis. 2020 Sep;11(9):1-12 IHC Human，Mouse 1:200 Nasopharyngeal carcinoma (NPC)tissue，NPI tissue，CNE1 cell-Xenograft

Activation of Autophagy Contributes to Sevoflurane-Induced Neurotoxicity in Fetal Rats. Frontiers in Molecular Neuroscience 2017 Dec 22 IF Rat 1:200 brain

Inhibition of Siah2 ubiquitin ligase ameliorates monocrotaline-induced pulmonary arterial remodeling through inactivation of YAP. LIFE SCIENCES Life Sci. 2020 Feb;242:117159 ICC Rat 1:200 PASMCs

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines. PLoS One Plos One. 2017 May;12(5):e0176823 IHC Mouse 1:200 skin

Activation of mTORC1 in subchondral bone preosteoblasts promotes osteoarthritis by stimulating bone sclerosis and secretion of CXCL12. Bone Research Bone Res. 2019 Feb;7(1):1-13 IHC Mouse Knee joint

Activation of mTORC1 in subchondral bone preosteoblasts promotes osteoarthritis by stimulating bone sclerosis and secretion of CXCL12. Bone Research Bone Res. 2019 Feb;7(1):1-13 IHC Mouse Knee joint

Activation of Autophagy Induces Monocrotaline-Induced Pulmonary Arterial Hypertension by FOXM1-Mediated FAK Phosphorylation LUNG Shaojun Li IHC Rat

Single-cell transcriptomic characterization of sheep conceptus elongation and implantation. Qi-En Yang IF Sheep 1:200 blastocyst

Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells. Cuixia Di IHC Mouse 1:200 HeLa cell-xenograft

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs