HSP70 Monoclonal Antibody(3G10)

    • Catalog No.:YM3042
    • Applications:WB,IHC-p,IF
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • HSPA1L/HSPA1A
      • Protein Name:
      • Heat shock 70 kDa protein 1-like/Heat shock 70 kDa protein 1A/1B
      • Immunogen:
      • Synthetic Peptide of HSP70
      • Specificity:
      • The antibody detects endogenous HSP70 proteins.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • WB: 1:1000-2000 IF: 1:100-200 IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • MolecularWeight(Da):
      • 70375/70052
      • Observed Band(KD):
      • 70
      • Background:
      • heat shock protein family A (Hsp70) member 1 like(HSPA1L) Homo sapiens This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008],
      • Function:
      • function:In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.,induction:Not induced by heat shock.,similarity:Belongs to the heat shock protein 70 family.,tissue specificity:Expressed in spermatids.,
      • Subcellular Location:
      • zona pellucida receptor complex,nucleoplasm,mitochondrial matrix,cytosol,COP9 signalosome,cell body,blood microparticle,
      • Expression:
      • Stomach mucosa,Testis,
      • Products Images
      • Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23910)was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,HSP70 Monoclonal Antibody(3G10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Human-breast-cancer tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Mouse-lung tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Rat-testis tissue. 1,HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:2000.
      • IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
      • Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse mAb diluted at 1:500.
      • Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.