Strep-Tag II Monoclonal Antibody(7A10)

    • Catalog No.:YM3016
    • Applications:WB
    • Reactivity:Species independent
      • Target:
      • Strep-Tag II
      • Immunogen:
      • Synthetic Peptide of Strep-Tag II
      • Specificity:
      • The antibody detects Strep-Tag II fusion proteins.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:5000-10000 IP 1:50-200
      • Purification:
      • Recombinant Expression and Affinity purified
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Strep-Tag II;Strep Tag II;
      • Products Images
      • Whole extracts were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Strep-Tag Ⅱ(7A10)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Rosetta transfected with an empty expression vector Lane 2: Rosetta transfected with a Strep-TagⅡ-tagged MBP protein Predicted band size: 55kDa Observed band size: 55kDa
      • Strep-Tag Ⅱ was immunoprecipitated from Strep-Tag Ⅱ-MBP(Rosetta) lysate with anti-Strep-Tag Ⅱ antibody. Western blot was performed on the immunoprecipitate using anti-Strep-Tag Ⅱ antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG Light chain antibody. Lane 1: Strep-Tag Ⅱ-MBP(Rosetta) lysate Lane 2: anti-Strep-Tag Ⅱ antibody IP in Strep-Tag Ⅱ-MBP(Rosetta) lysate Lane 3: Mouse monoclonal IgG (MNH209) in Strep-Tag Ⅱ-MBP(Rosetta) lysate.
      • 1ug Strep II fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000