• Target：
• BRAP

• Fields：
• >>Ras signaling pathway

• Gene Name：
• brap

• Human Gene Id：
• 8315

• Human Swiss Prot No：
• Q7Z569

• Mouse Swiss Prot No：
• Q99MP8

• Immunogen：
• Purified recombinant human BRAP protein fragments expressed in E.coli.

• Specificity：
• This antibody detects endogenous levels of BRAP and does not cross-react with related proteins.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• wb dilution 1:1000

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• 3010002G07Rik;BRAP2;BRCA1 associated protein;EC 6.3.2.;Galectin 2 binding protein;IMP;Impedes mitogenic signal propagation;Renal carcinoma antigen NY REN 63;RING finger protein 52;RNF52;zgc:92894.

• Observed Band(KD)：
• 67kD

• Background：
• The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008],

• Function：
• function:Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 UBP-type zinc finger.,subunit:Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.,tissue specificity:Expressed in breast epithelial cell lines.,

• Subcellular Location：
• Cytoplasm .

• Expression：
• Expressed in breast epithelial cell lines.

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