• Target：
• HSP A5/GRP78

• Fields：
• >>Protein export;>>Protein processing in endoplasmic reticulum;>>Antigen processing and presentation;>>Thyroid hormone synthesis;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Lipid and atherosclerosis

• Gene Name：
• hspa5

• Human Gene Id：
• 3309

• Human Swiss Prot No：
• P11021

• Mouse Swiss Prot No：
• P20029

• Immunogen：
• Purified recombinant human BiP/GRP78 protein expressed in E.coli.

• Specificity：
• This antibody detects endogenous levels of BiP/GRP78 and does not cross-react with related proteins.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• wb 1:1000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• 78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP;D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78;Endoplasmic reticulum lumenal Ca2+ binding protein grp78;FLJ26106;Glucose Regulated Protein 78kDa;GRP 78;GRP-78;GRP78;GRP78_HUMAN;Heat shock 70 kDa protein 5;Heat Shock 70kDa Protein 5;Hsce70;HSPA 5;HSPA5;Immunoglobulin Heavy Chain Binding Protein;Immunoglobulin heavy chain-binding protein;mBiP;MIF2;Sez7.

• Observed Band(KD)：
• 78kD

• Background：
• The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010],

• Function：
• disease:Autoantigen in rheumatoid arthritis [MIM:180300].,function:Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with DNAJC1 (via J domain) (By similarity). Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A.,

• Subcellular Location：
• Endoplasmic reticulum lumen . Melanosome . Cytoplasm . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390). .

• Expression：
• Articular cartilage,Brain,Cajal-Retzius cell,Cervix carcino

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells

The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis. Nature Communications Nat Commun. 2019 Jul;10(1):1-15 IF Mouse 1:100 lung

The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability. STEM CELLS AND DEVELOPMENT Stem Cells Dev. 2020 May;29(10):667-677 WB Rat 1:3000 Bone marrow mesenchymal stem cells(BMMSCs )

Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell

Subcellular Targets of Zinc Oxide Nanoparticles During the Aging Process: Role of Cross-talk Between Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in the Genotoxic Response. TOXICOLOGICAL SCIENCES Toxicol Sci. 2019 Sep;171(1):159-171 WB Human,Hamster AL cell

Treponema pallidum recombinant protein Tp0768 enhances the ability of HUVECs to promote neutrophil chemotaxis through TLR2/ER stress signaling pathway JOURNAL OF LEUKOCYTE BIOLOGY Cao Ting WB Human 1:1000 human umbilical vein endothelial cells (HUVECs)

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