• Target：
• PA28γ

• Fields：
• >>Proteasome;>>Antigen processing and presentation;>>Hepatitis C

• Gene Name：
• PSME3

• Protein Name：
• Proteasome activator complex subunit 3

• Human Gene Id：
• 10197

• Human Swiss Prot No：
• P61289

• Mouse Gene Id：
• 19192

• Mouse Swiss Prot No：
• P61290

• Immunogen：
• Purified recombinant human PA28γ protein fragments expressed in E.coli.

• Specificity：
• PA28γ Monoclonal Antibody detects endogenous levels of PA28γ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:1000 - 1:2000. IF 1:100 - 1:500. Not yet tested in other applications.

• Purification：
• Affinity purification

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PSME3;Proteasome activator complex subunit 3;11S regulator complex subunit gamma;REG-gamma;Activator of multicatalytic protease subunit 3;Ki nuclear autoantigen;Proteasome activator 28 subunit gamma;PA28g;PA28gamma

• Molecular Weight(Da)：
• 30kD

• Background：
• The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) o

• Function：
• disease:Sera from patients with systemic lupus erythematosus often contain antibodies that react with the Ki antigen.,domain:The C-terminal sequences affect heptamer stability and proteasome affinity.,function:Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-TP53/p53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53/p53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.,induction:Up-regulated in thyroid carcinoma cells.,PTM:Phosphorylated by MAP3K3.,similarity:Belongs to the PA28 family.,subcellular lo

• Subcellular Location：
• Nucleus . Cytoplasm . Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. .

• Expression：
• B-cell,Embryonic kidney,Fetal brain,Human endometrium carcinoma cell line,L

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