• Target：
• RSK2

• Fields：
• >>MAPK signaling pathway;>>Oocyte meiosis;>>mTOR signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Insulin resistance;>>Yersinia infection;>>Chemical carcinogenesis - receptor activation

• Gene Name：
• RPS6KA3

• Protein Name：
• Ribosomal protein S6 kinase alpha-3

• Human Gene Id：
• 6197

• Human Swiss Prot No：
• P51812

• Mouse Swiss Prot No：
• P18654

• Immunogen：
• Purified recombinant fragment of human Rsk-2 expressed in E. Coli.

• Specificity：
• Rsk-2 Monoclonal Antibody detects endogenous levels of Rsk-2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RPS6KA3;ISPK1;MAPKAPK1B;RSK2;Ribosomal protein S6 kinase alpha-3;S6K-alpha-3;90 kDa ribosomal protein S6 kinase 3;p90-RSK 3;p90RSK3;Insulin-stimulated protein kinase 1;ISPK-1;MAP kinase-activated protein kinase 1b;MAPK-activated

• Molecular Weight(Da)：
• 84kD

• References：
• 1. Mol Cell. 2009 Jan 16;33(1):109-16.
  2. Cancer Res. 2009 May 15;69(10):4398-406.

• Background：
• ribosomal protein S6 kinase A3(RPS6KA3) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,disease:Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-386, as part of the activation process.,PTM:Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase

• Subcellular Location：
• Nucleus . Cytoplasm .

• Expression：
• Expressed in many tissues, highest levels in skeletal muscle.

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