N-cadherin Monoclonal Antibody

    • Catalog No.:YM0465
    • Applications:IHC;IF;FCM;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • N-cadherin
      • Fields:
      • >>Cell adhesion molecules;>>Arrhythmogenic right ventricular cardiomyopathy
      • Gene Name:
      • CDH2
      • Protein Name:
      • Cadherin-2
      • Human Gene Id:
      • 1000
      • Human Swiss Prot No:
      • P19022
      • Mouse Swiss Prot No:
      • P15116
      • Immunogen:
      • Purified recombinant fragment of human N-cadherin expressed in E. Coli.
      • Specificity:
      • N-cadherin Monoclonal Antibody detects endogenous levels of N-cadherin protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CDH2;CDHN;NCAD;Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD antigen CD325
      • References:
      • 1. J Biol Chem. 2007 Mar 16;282(11):8545-56.
        2. Mol Cell Biochem. 2007 Aug;302(1-2):19-26.
        3. Urol Oncol. 2010 Mar-Apr;28(2):180-8.
      • Background:
      • This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],
      • Function:
      • function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,
      • Subcellular Location:
      • Cell membrane ; Single-pass type I membrane protein . Cell membrane, sarcolemma . Cell junction . Cell surface . Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. .
      • Expression:
      • Brain,Epithelium,Liver,
      • Products Images
      • Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Liang Duan WB Human 1:1000 HCT116 cell,SW480 cell
      • Western Blot analysis using N-cadherin Monoclonal Antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
      • Immunohistochemistry analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D) with DAB staining using N-cadherin Monoclonal Antibody.
      • Immunofluorescence analysis of A431 cells using N-cadherin Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
      • Flow cytometric analysis of PC-2 cells using N-cadherin Monoclonal Antibody (green) and negative control (purple).