• Target：
• Mi2-α

• Gene Name：
• CHD3

• Protein Name：
• Chromodomain-helicase-DNA-binding protein 3

• Human Gene Id：
• 1107

• Human Swiss Prot No：
• Q12873

• Immunogen：
• Purified recombinant fragment of human Mi2-α expressed in E. Coli.

• Specificity：
• Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CHD3;Chromodomain-helicase-DNA-binding protein 3;CHD-3;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH

• Molecular Weight(Da)：
• 227kD

• References：
• 1. Virus Res. 2003 Dec;98(1):83-91.
  2. Mol Cell. 2004 Sep 24;15(6):853-65.
  3. J Biol Chem. 2008 Dec 12;283(50):34976-82.

• Background：
• This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],

• Function：
• disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,

• Subcellular Location：
• Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .

• Expression：
• Widely expressed.

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