• Target：
• LysRS

• Fields：
• >>Aminoacyl-tRNA biosynthesis

• Gene Name：
• KARS

• Protein Name：
• Lysyl-tRNA synthetase

• Human Gene Id：
• 3735

• Human Swiss Prot No：
• Q15046

• Mouse Swiss Prot No：
• Q99MN1

• Immunogen：
• Purified recombinant fragment of LysRS(aa90-174) expressed in E. Coli.

• Specificity：
• LysRS Monoclonal Antibody detects endogenous levels of LysRS protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KARS;KIAA0070;Lysine--tRNA ligase;Lysyl-tRNA synthetase;LysRS

• Molecular Weight(Da)：
• 68kD

• References：
• 1. J Biol Chem. 2000 Nov 10;275(45):35063-9.
  2. J Virol. 2001 Jun;75(11):5043-8.
  3. Biochem Biophys Res Commun. 2002 Feb 15;291(1):158-64.
  4. Cell. 2004 Oct 15;119(2):195-208.

• Background：
• Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,subunit:Homodimer (By similarity); also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.,

• Subcellular Location：
• [Isoform Cytoplasmic]: Cytoplasm, cytosol . Cytoplasm . Nucleus . Cell membrane ; Peripheral membrane protein . Secreted . Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739). .; [Isoform Mitochondrial]: Mitochondrion .

• Expression：
• Bone marrow,Brain,Cerebellum,Hepatoma,Placenta,

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