KLHL22 Monoclonal Antibody
- Catalog No.：YM0403
- Applications：WB;IF;ELISA
- Reactivity：Human
- Target：
- KLHL22
- Gene Name：
- KLHL22
- Protein Name：
- Kelch-like protein 22
- Human Gene Id：
- 84861
- Human Swiss Prot No：
- Q53GT1
- Mouse Swiss Prot No：
- Q99JN2
- Immunogen：
- Purified recombinant fragment of human KLHL22 expressed in E. Coli.
- Specificity：
- KLHL22 Monoclonal Antibody detects endogenous levels of KLHL22 protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Monoclonal, Mouse
- Dilution：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification：
- Affinity purification
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- KLHL22;Kelch-like protein 22
- Molecular Weight(Da)：
- 72kD
- References：
- 1. Gene. 1994 Jan 28;138(1-2):171-4.
2. Nat Genet. 2004 Jan;36(1):40-5.
3. Cell. 2009 Jul 23;138(2):389-403.
- Background：
- similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,
- Function：
- similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,
- Subcellular Location：
- Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Nucleus . Lysosome . Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478). Localizes to the nucleus upon amino acid starvation (PubMed:29769719). Relocalizes to the cytosol and associates with lysosomes when amino acids are available (PubMed:29769719). .
- Expression：
- Embryo,Kidney,Liver,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis using KLHL22 Monoclonal Antibody against mouse brain tissues lysate.
- Immunofluorescence analysis of U251 cells using KLHL22 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
