IKKα Monoclonal Antibody

    • Catalog No.:YM0361
    • Applications:WB,IF/ICC,FCM,ELISA
    • Reactivity:Human
      • Gene Name:
      • CHUK
      • Protein Name:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • Human Gene Id:
      • 1147
      • Human Swiss Prot No:
      • O15111
      • Mouse Swiss Prot No:
      • Q60680
      • Immunogen:
      • Purified recombinant fragment of human IKKα expressed in E. Coli.
      • Specificity:
      • IKKα Monoclonal Antibody detects endogenous levels of IKKα protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
      • References:
      • 1. Mol Cancer. 2010 Jan 5;9:1.
        2. J Infect Dis. 2010 May 1;201(9):1371-80.
      • Background:
      • conserved helix-loop-helix ubiquitous kinase(CHUK) Homo sapiens This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • Subcellular Location:
      • intracellular,nucleoplasm,cytoplasm,cytosol,IkappaB kinase complex,cytoplasmic side of plasma membrane,CD40 receptor complex,intracellular membrane-bounded organelle,
      • Expression:
      • Brain,Cervix carcinoma,Heart,Lymph,T-cell,
      • Products Images
      • Western Blot analysis using IKKα Monoclonal Antibody against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.
      • Immunofluorescence analysis of NIH/3T3 cells using IKKα Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
      • Flow cytometric analysis of A549 cells using IKKα Monoclonal Antibody (blue) and negative control (red).