• Target：
• ESET

• Fields：
• >>Lysine degradation;>>Metabolic pathways;>>Signaling pathways regulating pluripotency of stem cells

• Gene Name：
• SETDB1

• Protein Name：
• Histone-lysine N-methyltransferase SETDB1

• Human Gene Id：
• 9869

• Human Swiss Prot No：
• Q15047

• Mouse Swiss Prot No：
• O88974

• Immunogen：
• Purified recombinant fragment of human ESET expressed in E. Coli.

• Specificity：
• ESET Monoclonal Antibody detects endogenous levels of ESET protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SETDB1;KIAA0067;KMT1E;Histone-lysine N-methyltransferase SETDB1;ERG-associated protein with SET domain;ESET;Histone H3-K9 methyltransferase 4;H3-K9-HMTase 4;Lysine N-methyltransferase 1E;SET domain bifurcated 1

• Molecular Weight(Da)：
• 143kD

• References：
• 1. Proteomics. 2005 Sep;5(14):3589-99.
  2. Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5308-13.
  3. Mol Cell Biochem. 2007 Nov;305(1-2):35-44.

• Background：
• SET domain bifurcated 1(SETDB1) Homo sapiens This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011],

• Function：
• catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,domain:The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.,function:Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is depende

• Subcellular Location：
• Nucleus . Cytoplasm . Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. .

• Expression：
• Widely expressed. High expression in testis.

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