• Target：
• EphB2

• Fields：
• >>Axon guidance

• Gene Name：
• EPHB2

• Protein Name：
• Ephrin type-B receptor 2

• Human Gene Id：
• 2048

• Human Swiss Prot No：
• P29323

• Mouse Swiss Prot No：
• P54763

• Immunogen：
• Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.

• Specificity：
• EphB2 Monoclonal Antibody detects endogenous levels of EphB2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EPHB2;DRT;EPHT3;EPTH3;ERK;HEK5;TYRO5;Ephrin type-B receptor 2;Developmentally-regulated Eph-related tyrosine kinase;ELK-related tyrosine kinase;EPH tyrosine kinase 3;EPH-like kinase 5;EK5;hEK5;Renal carcinoma antigen NY-REN-47

• Molecular Weight(Da)：
• 117kD

• References：
• 1. Nat Genet. 2004 Sep;36(9):979-83.
  2. Pediatr Res. 2005 Apr;57(4):537-44.

• Background：
• This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple tr

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EPHB2 are involved in the development of prostate cancer metastasis to the brain [MIM:603688].,disease:Defects in EPHB2 are involved in the progression of prostate cancer [MIM:176807].,function:Receptor for members of the ephrin-B family. Acts as a tumor suppressor.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Binds RASGAP through the juxtamembrane tyrosi

• Subcellular Location：
• Cell membrane; Single-pass type I membrane protein. Cell projection, axon . Cell projection, dendrite .

• Expression：
• Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs