• Target：
• Bmi-1

• Fields：
• >>Signaling pathways regulating pluripotency of stem cells;>>Transcriptional misregulation in cancer;>>MicroRNAs in cancer

• Gene Name：
• BMI1

• Protein Name：
• Polycomb complex protein BMI-1

• Human Gene Id：
• 648

• Human Swiss Prot No：
• P35226

• Mouse Swiss Prot No：
• P25916

• Immunogen：
• Purified recombinant fragment of human Bmi-1 expressed in E. Coli.

• Specificity：
• Bmi-1 Monoclonal Antibody detects endogenous levels of Bmi-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BMI1;PCGF4;RNF51;Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51

• Molecular Weight(Da)：
• 37kD

• References：
• 1. Mol Cancer. 2009 Nov 10;8:98.
  2. Cancer Res. 2009 Dec 1;69(23):9090-5.

• Background：
• BMI1 proto-oncogene, polycomb ring finger(BMI1) Homo sapiens This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015],

• Function：
• disease:Cooperates with the MYC oncogene to produce B-lymphomas.,function:Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.,PTM:May be polyubiquitinated; which does not lead to proteasomal degradation.,similarity:Contains 1 RING-type zinc finger.,subunit:Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and R

• Subcellular Location：
• Nucleus . Cytoplasm .

• Expression：
• Epithelium,Erythrocyte,Muscle,Thymus,

CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Jan;24(1):618-631 WB,IHC Human 1:1000,1:300 lung cancer tissues NCI-H460 cell, A549 cell

FEN1 upregulation mediated by SUMO2 via antagonizing proteasomal degradation promotes hepatocellular carcinoma stemness Translational Oncology Zhenxiang Peng WB Human Huh7 cell

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