• Target：
• Beclin 1

• Fields：
• >>Autophagy - other;>>Mitophagy - animal;>>Autophagy - animal;>>Apoptosis - multiple species;>>Apelin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection

• Gene Name：
• BECN1

• Protein Name：
• Beclin-1

• Human Gene Id：
• 8678

• Human Swiss Prot No：
• Q14457

• Mouse Swiss Prot No：
• O88597

• Immunogen：
• Purified recombinant fragment of human Beclin-1 expressed in E. Coli.

• Specificity：
• Beclin-1 Monoclonal Antibody detects endogenous levels of Beclin-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BECN1;GT197;Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197

• Molecular Weight(Da)：
• 52kD

• References：
• 1. Autophagy. 2008 Oct 1;4(7):947-8.
  2. J Clin Invest. 2008 Jun;118(6):2190-9.

• Background：
• beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

• Function：
• function:Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.,similarity:Belongs to the beclin family.,subcellular location:Expressed in dendrites and cell bodies of cerebellar Purkinje cells.,subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 (By similarity). Interacts with BCL2 and BCL2L1.,tissue specificity:Ubiquitous.,

• Subcellular Location：
• Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .; [Beclin-1-C 35 kDa]: Mitochondrion . Nucleus . Cytoplasm .; [Beclin-1-C 37 kDa]: Mitochondrion .

• Expression：
• Ubiquitous.

Enhanced autophagy reveals vulnerability of P-gp mediated epirubicin resistance in triple negative breast cancer cells. APOPTOSIS Apoptosis. 2016 Apr;21(4):473-488 WB Human MDA-MB-231 cell

Shi, Yanan, et al. "Salvianolic acid B ameliorates hepatocyte lipid droplet accumulation via stimulation of autophagy." (2020).

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