E2F-1 (Acetyl Lys120) Polyclonal Antibody

    • Catalog No.：YK0074
    • Applications：WB;ELISA
    • Reactivity：Human:K120;Mouse:K115;Rat:K118
      • Target：
      • E2F-1
      • Fields：
      • >>Endocrine resistance;>>Cell cycle;>>Mitophagy - animal;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
      • Gene Name：
      • E2F1 RBBP3
      • Protein Name：
      • E2F transcription factor 1
      • Human Gene Id：
      • 1869
      • Human Swiss Prot No：
      • Q01094
      • Mouse Swiss Prot No：
      • Q61501
      • Immunogen：
      • Synthesized acetyl-peptide from human protein at AA range: 100-170
      • Specificity：
      • This antibody detects endogenous levels of E2F-1 at Human:K120;Mouse:K115;Rat:K118, It doesn't reacte with total protein.
      • Formulation：
      • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500-10000 ELISA: 1:10000
      • Purification：
      • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • E2F1 RBBP3
      • Observed Band(KD)：
      • 60kD
      • Background：
      • The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
      • Function：
      • function:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, ren
      • Subcellular Location：
      • Nucleus .
      • Expression：
      • Brain,Epithelium,Pancreas,Skin,
      • Products Images
      • Western Blot analysis of MOUSE-BRIAN cells using Antibody diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

      Attributes

      • Code：
      • YK0074
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 190 / 340 / 600
      • Delivery：
      • 2024-11-24
      Order

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