Cleaved-PAR-4 (G48) Polyclonal Antibody

    • Catalog No.:YC0072
    • Applications:WB;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • PAR-4
      • Fields:
      • >>Rap1 signaling pathway;>>Neuroactive ligand-receptor interaction;>>Complement and coagulation cascades;>>Platelet activation;>>Pathways in cancer
      • Gene Name:
      • F2RL3
      • Protein Name:
      • Proteinase-activated receptor 4
      • Human Gene Id:
      • 9002
      • Human Swiss Prot No:
      • Q96RI0
      • Mouse Swiss Prot No:
      • O88634
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PAR4. AA range:29-78
      • Specificity:
      • Cleaved-PAR-4 (G48) Polyclonal Antibody detects endogenous levels of fragment of activated PAR-4 protein resulting from cleavage adjacent to G48.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • F2RL3;PAR4;Proteinase-activated receptor 4;PAR-4;Coagulation factor II receptor-like 3;Thrombin receptor-like 3
      • Observed Band(KD):
      • 33kD
      • Background:
      • This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016],
      • Function:
      • function:Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.,PTM:A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.,
      • Subcellular Location:
      • Cell membrane; Multi-pass membrane protein.
      • Expression:
      • Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.
      • Products Images
      • Western Blot analysis of NIH-3T3 cells using Cleaved-PAR-4 (G48) Polyclonal Antibody
      • Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is blocked with the synthesized peptide.