CD8 a (ABT304) IHC kit_ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

CD8 a (ABT304) IHC kit

Fields：
>>Cell adhesion molecules;>>Antigen processing and presentation;>>Hematopoietic cell lineage;>>T cell receptor signaling pathway;>>Yersinia infection;>>Primary immunodeficiency

Protein Name：
alpha polypeptide (p32);CD_antigen=CD8a;CD8;CD8 antigen alpha polypeptide;CD8 antigen alpha polypeptide (p32);CD8 antigen, alpha polypeptide (p32);CD8a;CD8A antigen;CD8A molecule;CD8A_HUMAN;Leu2;Leu2

Specificity：
The antibody can specifically recognize human CD8 protein, including two typies of dimer: αβ heterodimer or αα homodimer.

Purification：
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

Other Name：
alpha polypeptide (p32);CD_antigen=CD8a;CD8;CD8 antigen alpha polypeptide;CD8 antigen alpha polypeptide (p32);CD8 antigen, alpha polypeptide (p32);CD8a;CD8A antigen;CD8A molecule;CD8A_HUMAN;Leu2;Leu2 T lymphocyte antigen;Ly 2;Ly 35;Ly B;Ly2;Ly3;Ly35;LyB;Lyt 2.1 lymphocyte differentiation antigen (AA at 100);LYT3;MAL;OKT8 T cell antigen;OTTHUMP00000160760;OTTHUMP00000160764;OTTHUMP00000203528;OTTHUMP00000203721;p32;T cell antigen Leu2;T cell co receptor;T lymphocyte differentiation antigen T8/Leu 2;T-cell surface glycoprotein CD8 alpha chain;T-cell surface glycoprotein Lyt 2;T-lymphocyte differentiation antigen T8/Leu-2;T8 T cell antigen;T8/Leu-2 T-lymphocyte differentiation antigen

Background：
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011],

Function：
disease:Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.,function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.,online information:CD8 entry,online information:CD8A mutation db,PTM:All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Sho

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (YM6994), Anti-CD8 (YM6938), Anti-MCM2 (YM6077). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit (RS0039, Immunoway). The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (YM6994 1:200), Anti-CD8 (YM6938 1:200), Anti-MCM2 (YM6077 1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (YM6994), Anti-CD8 (YM6938), Anti-Ki-67 (YM6812). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit (RS0039, Immunoway). The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (YM6994 1:200), Anti-CD8 (YM6938 1:200), Anti-Ki67 (YM6812 1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Podoplanin (YM6994), Anti-CD8 (YM6938), Anti-MCM2 (YM6077). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit (RS0039, Immunoway). The section was incubated in 3 rounds of staining; sequentially for Anti-Podoplanin (YM6994 1:200), Anti-CD8 (YM6938 1:200), Anti-MCM2 (YM6077 1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).